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pet22b expression vector  (Millipore)


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    Structured Review

    Millipore pet22b expression vector
    Pet22b Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet22b+expression+vector/pet22b/pm38470050-311-22-25
    Average 90 stars, based on 1 article reviews
    pet22b expression vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Novel Klebsiella pneumoniae K23-Specific Bacteriophages From Different Families: Similarity of Depolymerases and Their Therapeutic Potential
    Article Snippet: .. Amplified fragments were cloned into a pET22b expression vector (Novagen, United States) via the NcoI and XhoI restriction sites and then transformed into Escherichia coli BL21 (DE3). ..

    Article Title: Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore
    Article Snippet: The algal strain S. elongatus PCC 7942 was purchased from the Freshwater Algae Culture Collection at the Institute of Hydrobiology, Wuhan, China. .. The SephrA and SephrB genes were amplified from the genomic DNA of S. elongatus PCC 7942, and cloned into the pET22b expression vector (Novagen) to obtain pETSePhrA and pETSePhrB plasmids, respectively. ..

    Article Title: Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number control
    Article Snippet: The DNA sequence for S. cerevisiae Ty1A (Uniprot P08405), codon-optimised for expression in E. coli , was synthesised by GeneArt. .. Sequences corresponding to residues M249-N355 (p18m AUG1 ) and M259-N355 (p18m AUG2 ) were amplified by PCR and the products were inserted into a pET22b expression vector (Novagen) between the NdeI and XhoI restriction sites in order to produce C-terminal fusion proteins containing the hexa-histidine tag PLEHHHHHH. .. Mutations were introduced into these parent constructs using the Quikchange II XL site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions.

    Clone Assay:

    Article Title: Novel Klebsiella pneumoniae K23-Specific Bacteriophages From Different Families: Similarity of Depolymerases and Their Therapeutic Potential
    Article Snippet: .. Amplified fragments were cloned into a pET22b expression vector (Novagen, United States) via the NcoI and XhoI restriction sites and then transformed into Escherichia coli BL21 (DE3). ..

    Article Title: Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore
    Article Snippet: The algal strain S. elongatus PCC 7942 was purchased from the Freshwater Algae Culture Collection at the Institute of Hydrobiology, Wuhan, China. .. The SephrA and SephrB genes were amplified from the genomic DNA of S. elongatus PCC 7942, and cloned into the pET22b expression vector (Novagen) to obtain pETSePhrA and pETSePhrB plasmids, respectively. ..

    Article Title: Biochemical characterization and gene structure analysis of the 24‐k D a glutathione transferase sigma from Taenia solium
    Article Snippet: .. The Ts24gst cDNA coding region was cloned into a pET22b expression vector (Novagen, Madison, WI, USA) and transformed in Escherichia coli BL21 (DE3) (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Biochemical characterization and gene structure analysis of the 24-kDa glutathione transferase sigma from Taenia solium.
    Article Snippet: .. Bacterial expression and protein purification The Ts24gst cDNA coding region was cloned into a pET22b expression vector (Novagen, Madison, WI, USA) and transformed in Escherichia coli BL21 (DE3) (Invitrogen, Carlsbad, CA, USA). ..

    Expressing:

    Article Title: Novel Klebsiella pneumoniae K23-Specific Bacteriophages From Different Families: Similarity of Depolymerases and Their Therapeutic Potential
    Article Snippet: .. Amplified fragments were cloned into a pET22b expression vector (Novagen, United States) via the NcoI and XhoI restriction sites and then transformed into Escherichia coli BL21 (DE3). ..

    Article Title: Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore
    Article Snippet: The algal strain S. elongatus PCC 7942 was purchased from the Freshwater Algae Culture Collection at the Institute of Hydrobiology, Wuhan, China. .. The SephrA and SephrB genes were amplified from the genomic DNA of S. elongatus PCC 7942, and cloned into the pET22b expression vector (Novagen) to obtain pETSePhrA and pETSePhrB plasmids, respectively. ..

    Article Title: Tetramerization is essential for the enzymatic function of the Pseudomonas aeruginosa virulence factor UDP-glucose pyrophosphorylase.
    Article Snippet: .. The PCR product was digested via BamHI and XhoI restriction sites introduced via primers and ligated into a likewise digested, previously modified pET22b expression vector (Novagen) containing an N-terminal Strep-tagII followed by April 2024 Volume 15 Issue 4 10.1128/mbio.02114-2317 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m bi o on 1 1 A pr il 20 24 b y 13 6. ..

    Article Title: Biochemical characterization and gene structure analysis of the 24‐k D a glutathione transferase sigma from Taenia solium
    Article Snippet: .. The Ts24gst cDNA coding region was cloned into a pET22b expression vector (Novagen, Madison, WI, USA) and transformed in Escherichia coli BL21 (DE3) (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number control
    Article Snippet: The DNA sequence for S. cerevisiae Ty1A (Uniprot P08405), codon-optimised for expression in E. coli , was synthesised by GeneArt. .. Sequences corresponding to residues M249-N355 (p18m AUG1 ) and M259-N355 (p18m AUG2 ) were amplified by PCR and the products were inserted into a pET22b expression vector (Novagen) between the NdeI and XhoI restriction sites in order to produce C-terminal fusion proteins containing the hexa-histidine tag PLEHHHHHH. .. Mutations were introduced into these parent constructs using the Quikchange II XL site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions.

    Article Title: helixCAM: A platform for programmable cellular assembly in bacteria and human cells.
    Article Snippet: Plasmids for E. coli expression of His-tagged coiled-coil peptides was done by first synthesizing codon-optimized gBlocks (IDT) comprising each Coiled-Coil with a C-terminal 6xHis tag. .. The DNA fragments were inserted into a pET22b expression vector (Novagen 69,744) via Gibson assembly (New England Biolabs E2611S). .. Plasmids for expression of His-tagged CC-GFP fusion protein were done by replacing the CMV promoter of the pcDNA3.1 vector (ThermoFisher V79020) with the CAG promoter, followed by insertion of the coiled-coil gBlock (codon-optimized formammalian expression) N-terminally to a TEV cleavage sequence, GFP, and 6xHis fusion (Figure S1). helixCAM K562 Cell line construction PiggyBac-flanked donor plasmids were packaged with the Super PiggyBac transposase vector (SBI PB210PA-1) using Lipofectamine 2000 (ThermoFisher 11,668,019) at a 1:1 ratio in Opti-MEM (ThermoFisher 31,985,062).

    Article Title: Biochemical characterization and gene structure analysis of the 24-kDa glutathione transferase sigma from Taenia solium.
    Article Snippet: .. Bacterial expression and protein purification The Ts24gst cDNA coding region was cloned into a pET22b expression vector (Novagen, Madison, WI, USA) and transformed in Escherichia coli BL21 (DE3) (Invitrogen, Carlsbad, CA, USA). ..

    Transformation Assay:

    Article Title: Novel Klebsiella pneumoniae K23-Specific Bacteriophages From Different Families: Similarity of Depolymerases and Their Therapeutic Potential
    Article Snippet: .. Amplified fragments were cloned into a pET22b expression vector (Novagen, United States) via the NcoI and XhoI restriction sites and then transformed into Escherichia coli BL21 (DE3). ..

    Article Title: Biochemical characterization and gene structure analysis of the 24‐k D a glutathione transferase sigma from Taenia solium
    Article Snippet: .. The Ts24gst cDNA coding region was cloned into a pET22b expression vector (Novagen, Madison, WI, USA) and transformed in Escherichia coli BL21 (DE3) (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Biochemical characterization and gene structure analysis of the 24-kDa glutathione transferase sigma from Taenia solium.
    Article Snippet: .. Bacterial expression and protein purification The Ts24gst cDNA coding region was cloned into a pET22b expression vector (Novagen, Madison, WI, USA) and transformed in Escherichia coli BL21 (DE3) (Invitrogen, Carlsbad, CA, USA). ..

    Periodic Counter-current Chromatography:

    Article Title: Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore
    Article Snippet: The algal strain S. elongatus PCC 7942 was purchased from the Freshwater Algae Culture Collection at the Institute of Hydrobiology, Wuhan, China. .. The SephrA and SephrB genes were amplified from the genomic DNA of S. elongatus PCC 7942, and cloned into the pET22b expression vector (Novagen) to obtain pETSePhrA and pETSePhrB plasmids, respectively. ..

    Plasmid Preparation:

    Article Title: Identification and characterization of a prokaryotic 6-4 photolyase from Synechococcus elongatus with a deazariboflavin antenna chromophore
    Article Snippet: The algal strain S. elongatus PCC 7942 was purchased from the Freshwater Algae Culture Collection at the Institute of Hydrobiology, Wuhan, China. .. The SephrA and SephrB genes were amplified from the genomic DNA of S. elongatus PCC 7942, and cloned into the pET22b expression vector (Novagen) to obtain pETSePhrA and pETSePhrB plasmids, respectively. ..

    Article Title: Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number control
    Article Snippet: The DNA sequence for S. cerevisiae Ty1A (Uniprot P08405), codon-optimised for expression in E. coli , was synthesised by GeneArt. .. Sequences corresponding to residues M249-N355 (p18m AUG1 ) and M259-N355 (p18m AUG2 ) were amplified by PCR and the products were inserted into a pET22b expression vector (Novagen) between the NdeI and XhoI restriction sites in order to produce C-terminal fusion proteins containing the hexa-histidine tag PLEHHHHHH. .. Mutations were introduced into these parent constructs using the Quikchange II XL site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Tetramerization is essential for the enzymatic function of the Pseudomonas aeruginosa virulence factor UDP-glucose pyrophosphorylase.
    Article Snippet: .. The PCR product was digested via BamHI and XhoI restriction sites introduced via primers and ligated into a likewise digested, previously modified pET22b expression vector (Novagen) containing an N-terminal Strep-tagII followed by April 2024 Volume 15 Issue 4 10.1128/mbio.02114-2317 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m bi o on 1 1 A pr il 20 24 b y 13 6. ..

    Article Title: Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number control
    Article Snippet: The DNA sequence for S. cerevisiae Ty1A (Uniprot P08405), codon-optimised for expression in E. coli , was synthesised by GeneArt. .. Sequences corresponding to residues M249-N355 (p18m AUG1 ) and M259-N355 (p18m AUG2 ) were amplified by PCR and the products were inserted into a pET22b expression vector (Novagen) between the NdeI and XhoI restriction sites in order to produce C-terminal fusion proteins containing the hexa-histidine tag PLEHHHHHH. .. Mutations were introduced into these parent constructs using the Quikchange II XL site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions.

    Modification:

    Article Title: Tetramerization is essential for the enzymatic function of the Pseudomonas aeruginosa virulence factor UDP-glucose pyrophosphorylase.
    Article Snippet: .. The PCR product was digested via BamHI and XhoI restriction sites introduced via primers and ligated into a likewise digested, previously modified pET22b expression vector (Novagen) containing an N-terminal Strep-tagII followed by April 2024 Volume 15 Issue 4 10.1128/mbio.02114-2317 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m bi o on 1 1 A pr il 20 24 b y 13 6. ..

    Synthesized:

    Article Title: Bacteriophage endolysin Ply113 as a potent antibacterial agent against polymicrobial biofilms formed by enterococci and Staphylococcus aureus
    Article Snippet: Molecular models were analyzed and presented by PyMOL (DeLano Scientific, Palo Alto, CA, United States). .. The gene fragment encoding Ply113 (GenBank Accession No. MZ147816.1) was synthesized by BGI Biotech (Beijing, China) and cloned into the pET22b expression vector (Novagen, San Diego, CA, USA). ..

    Protein Purification:

    Article Title: Biochemical characterization and gene structure analysis of the 24-kDa glutathione transferase sigma from Taenia solium.
    Article Snippet: .. Bacterial expression and protein purification The Ts24gst cDNA coding region was cloned into a pET22b expression vector (Novagen, Madison, WI, USA) and transformed in Escherichia coli BL21 (DE3) (Invitrogen, Carlsbad, CA, USA). ..



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